4 January 2022 – “Journal of Advanced Research” publishes article titled Raman-based spectrophenotyping of the most important cells of the immune system by: AleksandraBorek-Dorosz, Anna Maria Nowakowska, Patrycja Leszczenko, Adriana Adamczyk, Anna Pieczara, Justyna Jakubowska, Agata Pastorczak, Kinga Ostrowska, Marta Ząbczyńska, Karol Sowinski, Wieslaw I. Gruszecki, Malgorzata Baranska, Katarzyna Maria Marzec, and Katarzyna Majzner.
Quantification, analysis and recognition of biochemical features of T and B lymphocytes isolated from human blood was possible using label-free Raman spectroscopy imaging. That proves that this methodical approach can support the initial diagnosis or tracking the clinical course of several human diseases. We have developed a detailed protocol for measurement, data collection, pre-processing and analysis, which allow for differentiation of PBMCs populations obtained from a statistically significant number of healthy donors. Reliable discrimination of cells is possible based on spectral markers of carotenoids, nucleic acids, proteins and lipids, detected at the sub-cellular level.
Read the full article: https://www.sciencedirect.com/science/article/pii/S2090123221002666#f0045